C-Terminus
C-Terminus — The end of a peptide or protein chain terminated by a free carboxyl group (-COOH). Modifications at this position can alter stability and activity.
What Is the C-Terminus?
The C-terminus (carboxyl terminus) is the end of a peptide chain bearing a free carboxyl group (-COOH). Peptide sequences are read from N-terminus to C-terminus. In SPPS, the first residue attached to the resin becomes the C-terminal residue, and synthesis proceeds C-to-N.
C-Terminal Modifications
- Amidation (-NH2): Blocks carboxypeptidase degradation. Mimics natural PTM
- Acid (-OH): Free carboxyl. Default from Fmoc SPPS on Wang resin
- Thioester: C-terminal thioester for native chemical ligation
Frequently Asked Questions
What is C-Terminus?
The end of a peptide or protein chain terminated by a free carboxyl group (-COOH). Modifications at this position can alter stability and activity.
Why is C-Terminus important in peptide research?
C-Terminus is a fundamental concept in biochemistry as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.