Glossary

Amino Terminus

Biochemistry

Amino Terminus — The end of a peptide chain bearing the free alpha-amino group, also designated as the N-terminus, where peptide sequencing traditionally begins.

What Is the Amino Terminus?

The amino terminus (N-terminus) is the end of a peptide chain with a free amino group (-NH2 or -NH3+ at physiological pH). By convention, peptide sequences are written and read from N-terminus to C-terminus, and residues are numbered starting from the N-terminal residue as position 1. The N-terminus is the starting point for Edman sequencing.

N-Terminal Modifications

Acetylation: Blocks aminopeptidase degradation and neutralizes the positive charge
Pyroglutamate: Spontaneous cyclization of N-terminal Gln or Glu. Common PTM
Biotinylation: Biotin added to N-terminus for detection and capture
PEGylation: N-terminal PEG conjugation for half-life extension

Frequently Asked Questions

What is Amino Terminus?

The end of a peptide chain bearing the free alpha-amino group, also designated as the N-terminus, where peptide sequencing traditionally begins.

Why is Amino Terminus important in peptide research?

Amino Terminus is a fundamental concept in biochemistry as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.

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