BSA Conjugate
BSA Conjugate — A peptide covalently linked to bovine serum albumin, typically used as an immunogen to generate anti-peptide antibodies in research animals.
What Is a BSA Conjugate?
A BSA conjugate is a peptide covalently attached to bovine serum albumin for immunization, ELISA coating, or assay development. Short peptides (< 15 residues) are typically too small to be immunogenic alone and require conjugation to BSA or KLH as carrier proteins to elicit an antibody response.
Conjugation Methods
- EDC/sulfo-NHS: Crosslinks peptide N-terminus or Lys to BSA carboxyls
- Maleimide-Cys: Add C-terminal Cys to peptide, conjugate via sulfo-SMCC to BSA
- Glutaraldehyde: Simple but non-specific crosslinking of amines
Frequently Asked Questions
What is BSA Conjugate?
A peptide covalently linked to bovine serum albumin, typically used as an immunogen to generate anti-peptide antibodies in research animals.
Why is BSA Conjugate important in peptide research?
BSA Conjugate is a fundamental concept in laboratory as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.