Bovine Serum Albumin (BSA)
Bovine Serum Albumin (BSA) — A protein commonly used as a blocking agent in immunoassays, a standard for protein concentration assays, and a carrier protein in peptide studies.
What Is BSA?
Bovine serum albumin (BSA, 66 kDa) is the most commonly used protein standard and blocking agent in peptide research. BSA serves as a carrier protein for peptide immunization, a blocking agent in ELISA and Western blot, a stabilizer in dilute peptide solutions, and a MW standard in SEC.
Uses
- Blocking: 1-5% BSA blocks non-specific adsorption to plates and membranes
- Carrier: Peptide-BSA conjugates for immunization (generate anti-peptide antibodies)
- Stabilizer: 0.1% BSA in assay buffers prevents peptide adsorption to tubes and tips
- Standard: BCA/Bradford assay standard curve; SEC MW calibrant (66 kDa)
Frequently Asked Questions
What is Bovine Serum Albumin (BSA)?
A protein commonly used as a blocking agent in immunoassays, a standard for protein concentration assays, and a carrier protein in peptide studies.
Why is Bovine Serum Albumin (BSA) important in peptide research?
Bovine Serum Albumin (BSA) is a fundamental concept in reagent as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.
Authority Sources
- Bovine Serum Albumin (BSA) on Wikipedia
- Search Bovine Serum Albumin (BSA) on PubChem (NIH)
- Research articles on ScienceDirect