Glossary

Cyclic AMP (cAMP)

Biochemistry

Cyclic AMP (cAMP) — A second messenger molecule derived from ATP that mediates intracellular signaling downstream of many peptide hormone receptors.

What Is Cyclic AMP?

Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger generated by adenylyl cyclase upon Gs-coupled GPCR activation. cAMP activates protein kinase A (PKA), EPAC, and cyclic nucleotide-gated channels. cAMP assays are the primary readout for measuring activity of peptides targeting Gs- or Gi-coupled receptors.

cAMP Assays for Peptide Screening

HTRF/AlphaScreen: Competitive immunoassay. High-throughput 384/1536-well format
GloSensor: cAMP-dependent luciferase reporter. Real-time kinetic measurement
Gs agonists: CJC-1295 (GHRH-R), GLP-1 (GLP-1R) stimulate cAMP
Gi agonists: Somatostatin (SSTR) inhibits cAMP

Frequently Asked Questions

What is Cyclic AMP (cAMP)?

A second messenger molecule derived from ATP that mediates intracellular signaling downstream of many peptide hormone receptors.

Why is Cyclic AMP (cAMP) important in peptide research?

Cyclic AMP (cAMP) is a fundamental concept in biochemistry as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.

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