Sandwich Assay
Sandwich Assay — An immunoassay format using two antibodies to capture and detect a peptide antigen, providing high specificity and sensitivity.
What Is a Sandwich Assay?
A sandwich assay uses two antibodies binding different epitopes on the same peptide: a capture antibody immobilized on a plate and a detection antibody in solution. The peptide is "sandwiched" between them. Sandwich ELISAs are the gold standard for quantifying peptide hormones in biological samples due to high specificity and sensitivity.
Advantages
- Specificity: Dual antibody recognition virtually eliminates cross-reactivity
- Sensitivity: pg/mL detection. 10-100x more sensitive than competitive formats
- Requirement: Peptide must have ≥2 non-overlapping epitopes (minimum ~8-10 residues)
- Limitation: Small peptides (< 8 residues) may be too small for sandwich format
Frequently Asked Questions
What is Sandwich Assay?
An immunoassay format using two antibodies to capture and detect a peptide antigen, providing high specificity and sensitivity.
Why is Sandwich Assay important in peptide research?
Sandwich Assay is a fundamental concept in analytical as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.
Authority Sources
- Sandwich Assay on Wikipedia
- Search Sandwich Assay on PubChem (NIH)
- Research articles on ScienceDirect