Pyrogen
Pyrogen — Any substance that causes fever when introduced into the body, including bacterial endotoxins that must be excluded from research-grade peptide preparations.
What Is a Pyrogen?
A pyrogen is any substance that causes fever when introduced into the body. Bacterial endotoxin (LPS) is the most common and potent pyrogen. Pyrogen testing is mandatory for all injectable peptide products. Depyrogenation of glassware (250°C dry heat for 30 min) and endotoxin-free water are essential for pyrogen-free peptide preparation.
Testing
- LAL test: Limulus Amebocyte Lysate detects endotoxin specifically. < 0.25 EU/mL for injection water
- Rabbit pyrogen test: Historic standard. Measures temperature rise after IV injection
- MAT: Monocyte activation test detects non-endotoxin pyrogens as well
Frequently Asked Questions
What is Pyrogen?
Any substance that causes fever when introduced into the body, including bacterial endotoxins that must be excluded from research-grade peptide preparations.
Why is Pyrogen important in peptide research?
Pyrogen is a fundamental concept in quality as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.