Glossary

Percent Recovery

Analytical

Percent Recovery — The proportion of an analyte recovered after a sample preparation or purification process, a measure of method efficiency.

What Is Percent Recovery?

Percent recovery is the ratio of the amount of peptide recovered after a process step to the amount of peptide input, expressed as a percentage. It is the practical measure of process efficiency at every stage: cleavage from resin, HPLC purification, lyophilization, reconstitution, and sample preparation.

Typical Recovery Targets

SPPS cleavage: > 90% crude recovery from resin
Preparative HPLC: 50-80% recovery of target peptide at > 95% purity
Lyophilization: > 95% recovery (losses from vial adsorption)
SPE cleanup: > 70% recovery for most peptide SPE methods
Overall synthesis: 10-30% from resin to final lyophilized product for typical 20-30 residue peptides

Frequently Asked Questions

What is Percent Recovery?

The proportion of an analyte recovered after a sample preparation or purification process, a measure of method efficiency.

Why is Percent Recovery important in peptide research?

Percent Recovery is a fundamental concept in analytical as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.

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