Glossary

Palmitoylation

Glossary / Palmitoylation
Modification

Palmitoylation — The covalent attachment of palmitic acid to a cysteine residue, anchoring peptides to cell membranes and modulating protein trafficking.

Category
Modification
Glossary Section
P

What Is Palmitoylation?

Palmitoylation is the reversible attachment of palmitic acid (C16:0) to cysteine side chains via a thioester bond (S-palmitoylation). It is the most common lipid modification of intracellular proteins, anchoring them to membranes and regulating trafficking, signaling, and protein-protein interactions.

Peptide Applications

  • Lipidation strategy: Synthetic N-terminal palmitoylation increases peptide membrane interaction and cellular uptake
  • Self-adjuvanting vaccines: Pam2Cys and Pam3Cys lipopeptide adjuvants activate TLR2
  • Half-life extension: Palmitoylated peptides bind serum albumin (weaker than C18 fatty acids)

Frequently Asked Questions

What is Palmitoylation?

The covalent attachment of palmitic acid to a cysteine residue, anchoring peptides to cell membranes and modulating protein trafficking.

Why is Palmitoylation important in peptide research?

Palmitoylation is a fundamental concept in modification as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.

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