MALDI-TOF
MALDI-TOF — Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry, used for rapid molecular weight determination of peptides and proteins.
What Is MALDI-TOF?
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry ionizes peptides from a crystalline matrix by pulsed laser irradiation, then measures their mass by time of flight. MALDI-TOF produces predominantly singly charged ions ([M+H]⁺), giving simple spectra ideal for rapid peptide MW confirmation.
Applications
- MW confirmation: Rapid (<1 min) identity check for synthetic peptides. Mass accuracy ±0.01%
- Peptide mapping: Enzymatic digest spotted on MALDI plate for fingerprint analysis
- Imaging: MALDI-MSI maps peptide distribution in tissue sections at 10-50 µm resolution
- Matrix: α-CHCA for peptides < 5 kDa; sinapinic acid for proteins > 5 kDa
Frequently Asked Questions
What is MALDI-TOF?
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry, used for rapid molecular weight determination of peptides and proteins.
Why is MALDI-TOF important in peptide research?
MALDI-TOF is a fundamental concept in analytical as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.