Expression Vector
Expression Vector — A DNA construct containing regulatory elements that drive the production of a recombinant peptide in a host organism such as E. coli or yeast.
What Is an Expression Vector?
An expression vector is a plasmid or viral DNA construct engineered to produce a recombinant peptide in a host cell. Expression vectors contain a promoter (drives transcription), ribosome binding site, the peptide-encoding gene, a selection marker (antibiotic resistance), and often a fusion tag for purification.
Components
- Promoter: T7 (IPTG-inducible, E. coli), CMV (constitutive, mammalian), AOX1 (methanol, Pichia)
- Tag: His6, GST, MBP, SUMO for affinity purification and solubility enhancement
- Cleavage site: TEV, thrombin, or SUMO protease site between tag and peptide
Frequently Asked Questions
What is Expression Vector?
A DNA construct containing regulatory elements that drive the production of a recombinant peptide in a host organism such as E. coli or yeast.
Why is Expression Vector important in peptide research?
Expression Vector is a fundamental concept in manufacturing as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.
Authority Sources
- Expression Vector on Wikipedia
- Search Expression Vector on PubChem (NIH)
- Research articles on ScienceDirect