BPC-157
BPC-157 — Body Protection Compound-157, a synthetic pentadecapeptide derived from human gastric juice protein, studied extensively for its tissue-protective properties in vitro.
What Is BPC-157?
BPC-157 (Body Protection Compound-157) is a synthetic pentadecapeptide consisting of 15 amino acids. Its sequence (Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val) is derived from a protective protein found naturally in human gastric juice. Unlike many research peptides, BPC-157 is stable in gastric acid, which has made it a subject of extensive preclinical investigation.
The compound has a molecular weight of approximately 1419.53 Da and a molecular formula of C₆₂H₉₈N₁₆O₂₂. It is typically supplied as a white lyophilized powder with purity ≥98% as verified by HPLC.
Mechanism of Action
Research suggests BPC-157 operates through multiple molecular pathways. In vitro studies have identified interactions with the nitric oxide (NO) system, the FAK-paxillin signaling pathway, and the upregulation of growth hormone receptors. The peptide has demonstrated the ability to promote angiogenesis (formation of new blood vessels) in cell culture models and to modulate the expression of genes involved in extracellular matrix remodeling.
BPC-157 also appears to interact with the dopaminergic system and has shown effects on serotonin, GABA, and opioid receptor pathways in animal models. These multi-target interactions distinguish it from single-mechanism compounds and have generated significant research interest across multiple disciplines.
Key Research Findings
Published preclinical research on BPC-157 spans several areas:
- Tissue repair: Studies in rat models have shown accelerated healing of tendons, ligaments, muscle, and bone tissue (Seiwerth et al., Journal of Physiology Paris, 1999)
- Gastrointestinal protection: The peptide has demonstrated cytoprotective effects against NSAID-induced damage, alcohol-induced lesions, and inflammatory bowel conditions in animal models
- Neuroprotection: Research in rodent models has shown effects on dopaminergic neuron preservation and peripheral nerve regeneration
- Vascular effects: In vitro studies indicate promotion of endothelial cell migration and tube formation, suggesting angiogenic properties
It is important to note that all published research on BPC-157 is preclinical. No human clinical trials have been completed to date, and the compound is sold exclusively for laboratory research purposes.
Reconstitution and Storage
BPC-157 is supplied as a lyophilized powder and should be stored at -20°C prior to reconstitution. For reconstitution, add bacteriostatic water slowly along the vial wall to avoid disrupting the peptide cake. Do not vortex or shake vigorously.
Once reconstituted, the solution should be stored at 2-8°C and used within 30 days. For longer storage, aliquot into single-use portions and store at -20°C to minimize freeze-thaw cycles. Always calculate dosing based on net peptide content, not gross powder weight.
Quality Verification
When evaluating BPC-157 from any supplier, verify the following on the Certificate of Analysis:
- Purity: ≥98% by HPLC (check the chromatogram for a single dominant peak)
- Molecular weight: 1419.53 ± 0.5 Da confirmed by mass spectrometry
- Amino acid sequence: Confirmed by MS/MS fragmentation matching the published sequence
- Endotoxin levels: Below detection limits for cell culture applications
- Appearance: White to off-white lyophilized powder, no discoloration
Frequently Asked Questions
What is BPC-157?
Body Protection Compound-157, a synthetic pentadecapeptide derived from human gastric juice protein, studied extensively for its tissue-protective properties in vitro.
Why is BPC-157 important in peptide research?
BPC-157 is a fundamental concept in compound as it relates to peptide science. It directly influences experimental design, compound characterization, and the reliability of research outcomes across biochemistry and molecular biology disciplines.